Pharmaceutical Biotechnology Questions and Answers - Plasma Protein Drug Binding

1. Which one of the following is the correct order of the drugs binding to various plasma protein?
a) Albumin > alpha-1 acid glycoprotein > globulins > lipoproteins
b) Albumin > globulins > lipoproteins > alpha-1 acid glycoprotein
c) Albumin > alpha-1 acid glycoprotein > lipoproteins > globulins
d) Albumin > lipoproteins > globulins > alpha-1 acid glycoprotein

Answer: c
Explanation: The binding of the drug to the plasma protein is reversible. The extent of the binding is in the following order albumin > alpha-1 acid glycoprotein > lipoproteins > globulins. Drugs of all types, varieties can easily bind to the Human serum albumin.

2. Which vitamin molecules will bind to alpha 2 globulin?
a) Vitamin b complexes
b) Vitamin a and vitamin b
c) Vitamin A, D, E, K
d) Steroids bind to alpha 2 globulin, not vitamin

Answer: c
Explanation: Vitamin A, D, E, K are the vitamins that are fat soluble proteins. Thus these are the vitamins and also cupric ions which bind to alpha 2 globulin

3. What is the molecular weight of human serum albumin?
a) 5000 Dalton
b) 65,000 Dalton
c) 60,000 Dalton
d) 75,000 Dalton

Answer: b
Explanation: The human serum albumin is the most abundant protein in the human body. The molecular weight of HSA id 65,000Dalton. It has a large drug binding capacity. It can bind to several compounds with a variety of structure.

4. HSA comes under which protein family?
a) Globulin
b) Receptor
c) Enzymes
d) Catalyst

Answer: a
Explanation: The human serum albumin is the most abundant protein in the human body. It has a large drug binding capacity. It can bind to several compounds with a variety of structure. There are 4 different drugs binding site in HSA. It is of globulin family.

5. What is the name of the drug binding site I of HSA?
a) Tamoxifen binding site
b) Digitoxin binding site
c) Diazepam binding site
d) Warfarin and azapropazone binding site

Answer: d
Explanation: In the structure of HSA there 4 main binding sites for drugs. The site I am called as the warfarin and azapropazone binding site. In this region, a large number of drugs can bind. For example several NSAIDs, phenytoin, sodium valproate, bilirubin.

6. Which one of the below does not belong to the 4 classes of lipoprotein?
a) Chylomicrons
b) Very low-density lipoproteins
c) High-density lipoprotein
d) Fatty acids

Answer: d
Explanation: Lipoproteins are classified into 4 classes. The classes are chylomicrons, very low-density lipoproteins (VLDL), low-density lipoproteins (LDL), high-density lipoproteins (HDL)

7. Which of the given drugs bind to Α1 globulin?
a) Steroidal drugs
b) Vitamins A, D, E, K
c) Ferrous ions
d) Carotenoids

Answer: a
Explanation: Several plasma globulins were identified and named as Α1, Α2, Β1, Β2, γ globulins. Α1 globulin bind to steroidal drugs. For example cortisone, prednisone, thyroxine, cyanocobalamin. It is also known as transcortin.

8. What is the name of the drug binding site IV of HSA?
a) Tamoxifen binding site
b) Digitoxin binding site
c) Diazepam binding site
d) Warfarin and azapropazone binding site

Answer: a
Explanation: In the structure of HSA there 4 main binding sites for drugs. The 4th drug binding site name is Tamoxifen binding site.

9. Acidic drugs like imipramine, lidocaine bind to alpha 1 Acid Glycoprotein.
a) True
b) False

Answer: b
Explanation: Alpha 1 Acid Glycoprotein is also called as orsomucoid. It has a molecular weight of 44,000 and a plasma concentration of range 0.04-0.1%. It binds basic drugs like imipramine, lidocaine, quinidine, etc

10. Which of the given drugs bind to Β2 globulin?
a) Steroidal drugs
b) Vitamins A, D, E, K
c) Ferrous ions
d) Carotenoids

Answer: d
Explanation: Several plasma globulins were identified and named as Α1, Α2, Β1, Β2, γ globulins. Β2 globulin binds to carotenoids.