1. Cyclic Fed-Batch culture has been used for the production of _______
a) Bovine serum albumin
b) Penicillin
c) Human serum albumin
d) Cheese Production
Explanation: Cyclic fed‐batch culture has been used for the production of human serum albumin. Human Serum Albumin is an organic component of blood plasma, which is used to maintain osmotic pressure of blood.
2. What is the unit of substrate feed rate (G)?
a) dm3
b) dm3 h-1
c) g dm-3 h-1
d) g dm3
Explanation: The unit of substrate feed rate G is g dm-3 h-1 which is given by the equation,
(dx/dt) * Y = G where Y is the yield factor.
3. The feed solution in fed-batch culture is highly concentrated to _______
a) avoid dilution of the bioreactor
b) maintain low cell density in bioreactor
c) avoid lag phase
d) increase productivity
Explanation: The feed solution in fed-batch culture is highly concentrated to avoid dilution of the bioreactor and to reach high cell density in a bioreactor.
4. Which of the following is NOT a criterion for the choice of an organism?
a) The organism must be genetically stable
b) The organism must be able to produce a high yield of product
c) The optimum temperature for the growth of an organism must be above 50°C
d) The organism must be able to grow in an easily available nutrient medium
Explanation: The optimum temperature for the growth of an organism should be above 40°C. This reduces the cooling costs in large scale fermenters. The microorganisms are chosen based on the rest three criteria.
5. Full-form of ATCC is _________
a) American Type Culture Collection
b) Automatic Type Counter & Classifier
c) American Type Counter Collection
d) American Type Classifier and Collection
Explanation: ATCC stands for American Type Culture Collection. It is a non-profit organization and a key resource for medical research. It is the organization which has the largest department of Research and Development.
6. Which of the following method is not used in isolation and screening of desired microorganisms?
a) Crowded plate technique
b) Auxanographic technique
c) Enrichment Culture technique
d) Hanging Drop technique
Explanation: Hanging drop technique is used for motility testing of bacteria. The methods for isolation and screening are a crowded plate, auxanography, enrichment culture and use of indicator dyes.
7. The screening is isolation and detection of microorganisms of interest.
a) true
b) false
Explanation: Screening is the use of highly selective procedures to allow the detection and isolation of only those microorganisms of interest from a large microbial population.
8. Which of the following method is useful for the isolation and detection of organisms having the ability to produce antibiotics?
a) Crowded plate technique
b) Auxanographic technique
c) Enrichment Culture technique
d) Indicator dye technique
Explanation: The Crowded Plate Technique is used for the detection and isolation of the organisms which are antibiotic producers. The serial dilutions of soil are performed and spread on a Petri plate and allowed to grow. The well-isolated colonies are antibiotic producers with the zone of inhibition.
9. Which of the following shows the zone of inhibition when a particular organism is grown on a Petri plate?
a) Growth Factor producers
b) Antibiotic producers
c) Organic acid producers
d) Amino acid producers
Explanation: The Antibiotic producers show the zone of inhibition. It is the zone where only the growth of a particular colony is observed whereas rest all colonies get degraded by antibiotics.
10. Which of the following method is useful for isolation and detection of organisms having the ability to produce growth factors?
a) Crowded plate technique
b) Auxanographic technique
c) Enrichment Culture technique
d) Indicator dye technique
Explanation: The Auxanographic technique is used for the detection and isolation of the organisms which are growth factor producers. The growth factor-like amino acids, vitamins promotes the growth of auxotrophic mutants.