1. What type of mixture is collected in round bottom flask after passing through a steam generator?
a) Mixture of volatile organic compound and water
b) Mixture of nonvolatile organic compound and water
c) Mixture of nonvolatile inorganic compound and steam
d) Mixture of volatile organic compound and steam
Explanation: Mixture of volatile organic compound and steam is collected in round bottom flask after passing through steam generator.
2. Which of the following property is not possessed by the organic solvent required to separate organic compound with water?
a) It is immiscible with water
b) It is less volatile than water
c) It is easily vaporisable
d) Organic compound is more soluble in it
Explanation: Organic solvent required to separate organic compound with water is more volatile liquid than water. All the other mentioned properties are possessed by the organic solvent required to separate organic compound with water.
3. When does the organic compound be present in the upper solvent layer in differential extraction?
a) Before the extraction
b) During the extraction
c) After the extraction
d) It is always present in the upper layer
Explanation: The organic compound is present in the solvent layer after the extraction. Differential extraction refers to the process by which the DNA from two different types of cells can be extracted without mixing their contents
4. The organic compound in differential extraction is recovered from the organic solvent by ____________
a) Distillation
b) Fractional crystallisation
c) Steam crystallisation
d) Sublimation
Explanation: The organic compound in differential extraction is recovered from the organic solvent by distillation by evaporation.
5. Chromatography is the process for identification, purification and separation of components of a mixture on the basis of ____________
a) Difference in their boiling point
b) Difference in their melting point
c) Difference in their affinity for mobile and stationary phase
d) Difference in their solubility
Explanation: Chromatography is the process for identification, purification and separation of components of a mixture on the basis of difference in their affinity for mobile and stationary phases. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase.
6. Chromatography involves two mutually ____________
a) Immiscible phases
b) Miscible phases
c) Soluble phases
d) None of the mentioned
Explanation: Chromatography involves two mutually immiscible phases (stationary and mobile phases). The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase.
7. There is no need of support if packed into a column when the stationary phase is ____________
a) Gas
b) Liquid
c) Solid
d) None of the mentioned
Explanation: If the stationary phase is solid, there is no need of support if packed into a column. In solid phase, all the molecules are already in closed packing.
8. Which of the following is not a stationary phase?
a) Liquid-liquid chromatography
b) Gas-liquid chromatography
c) Gas-solid chromatography
d) Solid-solid chromatography
Explanation: Solid-solid chromatography is not a stationary phase because solid-solid phase cannot provide any fluidity.
9. Liquid chromatography is a technique for separating ____________
a) Ions that are not dissolved in a solvent
b) Ions that are dissolved in a solvent
c) Ions that are dissolved in a solute
d) All of the mentioned
Explanation: Liquid chromatography is a technique for separating ions that are dissolved in a solvent. Solute is the material which is dissolved and solvent is the solution in which it is dissolved.
10. In exclusion chromatography, solute molecules are separated based on ____________
a) Molecular geometry and size
b) Molecular composition
c) Molecular phase
d) Molecular formula
Explanation: In exclusion chromatography, solute molecules are separated based on molecular geometry and size. These two physical characteristics have a significance role in exclusion chromatography.